Background: Infertility defined by the failure to achieve pregnancy after 12 months or more of regular unprotected sexual intercourse. The electrochemiluminescence immunoassay is one methods intended to measure serum hormone levels. Aim: to assess the hormonal level using cobas e411 immunoassay analyzers. Subject and method: A case series study design was chosen to study 60 infertile women in the childbearing. The study was done at Privet Pharma Laboratory in Erbil for one-month duration during 2017. Using electrochemiluminescence immunoassay with Sandwich principle. Result: The study revealed that women with secondary infertility formed 56.7% and women with primary infertility formed 43.3%. Two third of women in age group (15-20) year were primary infertile and 81.9% of women in age group more than 35 year were secondary infertile. P-value 0.125 and 0.062 and respectively. Three forth of study sample had abnormal level of AMH, it was 17 (37%) among women with primary infertility and it was 29 (63%) among women with secondary infertility P- value 0.071. 68.4% of women with secondary infertility had Abnormal level FSH hormone if compeer with women with primary infertility, it was 31.6%. Although LH was abnormal level among women with primary infertility if it compared with women with secondary infertility but statistically not significant. There was not very big differences between infertile women and their normal and abnormal level of prolactin hormone Conclusion: Two third of infertile women in age group 15-20 year had primary infertility and four fifth of women in age ≥35 year had secondary infertility. Abnormal hormonal level among study sample were AMH, prolactin and LH.