Introduction: Hemolytic anemia is defined as anemia characterized by premature and excessive destruction of red blood cells. Several etiologies are at the root of these anemias, including sickle cell disease. In this work, we evaluated the incidence of sickle cell anemia in the hemolitic anemia in the city of Kinshasa in order to contribute to the improvement of the care of people suffering from anemia. Materials and methods: 512 patients with anemia from 0 to 37 years old, of whom 335 girls and 177 boys participated in the study. The anemia of the patients was confirmed by the hemogram. The haemolytic character of the anemia was confirmed by indirect bilirubin, lactate dehydrogenase assay and reticulocyte count. Sickle cell disease was diagnosed by isoelectric focusing electrophoresis. Results: Hemoglobin levels (6.1 +/- 2g / dl) and hematocrit (20.5 +/- 6.5%) achieved by the hemogram showed that all subjects had anemia. Levels of LDH (479,04 ± 76,79 IU/l), indirect bilirubin (21.06 ± 3.34 μg / l) and reticulocytes (231.118 / mm3) confirmed the haemolytic nature of anemia of patients in 465/512 is 90.8%. Isoelectric focusing electrophoresis revealed that 4.7% of the subjects were homozygous (SS); 7.3% of subjects were heterozygous (AS); 88% of the subjects were normal (AA). Conclusion: The study showed an incidence of sickle cell disease of 4.7% in hemolytic anemias in city of Kinshasa. The hemoglobinopathies including sickle cell disease must be taken into account in the diagnosis of haemolytic anemias in the city of Kinshasa province in particular and in DR Congo in general in order to improve the care of people suffering from anemia in DR Congo.