Background: Neonatal jaundice affects every second infants globally. Jaundice is the result of an accumulation of bilirubin due to immature neonate liver. High serum levels of bilirubin cause lethargy, poor feeding and kernicterus of the neonate. Aims and objectives: To study the prevalence of jaundice in neonates. Materials and Methods: Seven hundred and eighty two neonates were studied at the maternity ward of Taj Hospital and Dental clinic Agra Uttar Pradesh from May 2018 to May 2019. Serum bilirubin level was estimated in all the neonates. Results: Out of 782 newborns, 355 (45.39%) infants developed clinical jaundice. Of this 355, 216 (60.84%) developed the physiological jaundice and 139 (39.15%) had non-physiological jaundice needing therapeutic intervention. Conclusion: Incidence of jaundice is high among the neonates mainly the physiological jaundice.