Background: Research studies have demonstrated that yoga can modulate autonomic functions. Hence, the current study was designed to assess the effect of yoga on autonomic functions. Method: 100 healthy volunteers (146 M; 54 F) from age group (17-26) years were included in the study. They practiced some yogic exercises including Asanas, Pranayam & relaxation techniques daily one hour for 3 months. The autonomic function tests to measure the parasympathetic reactivity were deep breathing test (DBT), lying to standing test (LST) and valsalva ratio (VR). For sympathetic reactivity hand grip test (HGT) and cold pressure test (CPT) were performed. Result: Heart rate response to deep breathing (E: I ratio) increased significantly while 30:15 ratio during LST and valsalva ratio (VR) did not change significantly after the yoga training (P>0.05). In Isometric handgrip test (IHG test) rise in SBP (∆SBP) was not significantly altered after 3 months of yoga training whereas rise in DBP (∆DBP) was significantly decreased in response to IHG test. Range/response (∆) of systolic blood pressure and diastolic blood pressure during cold pressor test (CPT) was found to be significantly reduced Conclusion: These findings suggest that, Yoga training improved autonomic functions in healthy volunteers.