Aim: To evaluate GeneXpert MTB/RIF test results performed in a tertiary care hospital and analyse the incidence of multi-drug resistance. Method: Samples were processed by GeneXpert MTB/RIF assay which is an ultrasensitive hemi-nested PCR which simultaneously identifies M. tuberculosis and detect rifampicin resistance directly from clinical specimens. The samples were also subjected to ZN staining and the results were correlated. Result: From a total of 257 samples, 33 samples were positive for GeneXpert MTB/RIF and none of them showed rifampicin resistance in the study. BAL samples predominated among the types of samples 104 (40.46%). Males were high (60.7%) in the study and samples collected were found to be the highest among age group above 60 (15.95%). Rate of positivity was high among 41-50 age group (21.21%). 16 out of 33 positive samples failed to show positive results in ZN staining while 17 positive samples were positive for smear as well.