Comprehensive analysis of the discharge from the purulent wounds was carried out by bacteriological, PCR and GLC of methods. It was found that the greatest numbers of strains were presented obligate non-spore-forming anaerobic bacteria (34.6%), which prevailed over the facultative-anaerobic, aerobic forms and fundus (22.7%) for bacteriological examination. In normergic type of inflammatory reaction microbial associations were often presented by 4-6 species of microorganisms (61.3%), in hypergic type till 6-8 species (90.3%) and in hyperergic type with 3-4 species. PCR study was found higher percentages of microbial associations (Prevotella intermedia 79, 2%, Porph. Gingivalis 87, 5%) in comparison with bacteriological study (2.5% and 0.8%, respectively). The high correlation observed between microbiological and GLC and makes the detection all occurring markers of microorganisms - more than 0.9.